Performance evaluation of high-volume electret filter air samplers in aerosol microbiome research.

ABSTRACT:

Background

Reliable identification and quantification of bioaerosols is fundamental in aerosol microbiome research, highlighting the importance of using sampling equipment with well-defined performance characteristics. Following advances in sequencing technology, shotgun metagenomic sequencing (SMS) of environmental samples is now possible. However, SMS of air samples is challenging due to low biomass, but with the use of high-volume air samplers sufficient DNA yields can be obtained. Here we investigate the sampling performance and comparability of two hand-portable, battery-operated, high-volume electret filter air samplers, SASS 3100 and ACD-200 Bobcat, previously used in SMS-based aerosol microbiome research.

Results

SASS and Bobcat consistently delivered end-to-end samp

SUBMITTER: Boifot KO 

PROVIDER: S-EPMC8067322 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature