The Leloir Cycle in Glioblastoma: Galactose Scavenging and Metabolic Remodeling.

ABSTRACT:

Background

Glioblastoma (GBM) can use metabolic fuels other than glucose (Glc). The ability of GBM to use galactose (Gal) as a fuel via the Leloir pathway is investigated.

Methods

Gene transcript data were accessed to determine the association between expression of genes of the Leloir pathway and patient outcomes. Growth studies were performed on five primary patient-derived GBM cultures using Glc-free media supplemented with Gal. The role of Glut3/Glut14 in sugar import was investigated using antibody inhibition of hexose transport. A specific inhibitor of GALK1 (Cpd36) was used to inhibit Gal catabolism. Gal metabolism was examined using proton, carbon and phosphorous NMR spectroscopy, with ¹³C-labeled Glc and Gal as tracers.

Results

Data analysis from pu

SUBMITTER: Sharpe MA 

PROVIDER: S-EPMC8069026 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature