Luminescent Phage-Based Detection of Klebsiella pneumoniae: From Engineering to Diagnostics.

ABSTRACT: Bacteriophages ("phages") infect and multiply within specific bacterial strains, causing lysis of their target. Due to the specific nature of these interactions, phages allow a high-precision approach for therapy which can also be exploited for the detection of phage-sensitive pathogens associated with chronic diseases due to gut microbiome imbalance. As rapid phage-mediated detection assays becoming standard-of-care diagnostic tools, they will advance the more widespread application of phage therapy in a precision approach. Using a conventional method and a new cloning approach to develop luminescent phages, we engineered two phages that specifically detect a disease-associated microbial strain. We performed phage sensitivity assays in liquid culture and in fecal matrices and tested the s

SUBMITTER: Zelcbuch L 

PROVIDER: S-EPMC8069110 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature