Induction of foxp3 during the Crosstalk between Antigen Presenting Like-Cells MHCII⁺CD83⁺ and Splenocytes CD4⁺IgM^- in Rainbow Trout.

ABSTRACT: In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Using these splenocytes, co-cultures of 24 and 48 h are used to determine the gene expression of master transcriptional factors that coordinate the polarization of T cells (t-bet, gata3, and foxp3). The results show a proportional upregulation of fo

SUBMITTER: Morales-Lange B 

PROVIDER: S-EPMC8069158 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature