Stability of Imprinting and Differentiation Capacity in Naive Human Cells Induced by Chemical Inhibition of CDK8 and CDK19.

ABSTRACT: Pluripotent stem cells can be stabilized in vitro at different developmental states by the use of specific chemicals and soluble factors. The naïve and primed states are the best characterized pluripotency states. Naïve pluripotent stem cells (PSCs) correspond to the early pre-implantation blastocyst and, in mice, constitute the optimal starting state for subsequent developmental applications. However, the stabilization of human naïve PSCs remains challenging because, after short-term culture, most current methods result in karyotypic abnormalities, aberrant DNA methylation patterns, loss of imprinting and severely compromised developmental potency. We have recently developed a novel method to induce and stabilize naïve human PSCs that consists in the simple addition of a chemical inhibito

SUBMITTER: Bernad R 

PROVIDER: S-EPMC8069959 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature