Electrochemical DNA Biosensor That Detects Early Celiac Disease Autoantibodies.

ABSTRACT: Although it is estimated that more than one million Americans have celiac disease (CD), it remains challenging to diagnose. CD, an autoimmune and inflammatory response following the ingestion of gluten-containing foods, has symptoms overlapping with other diseases and requires invasive diagnostics. The gold standard for CD diagnosis involves serologic blood tests followed by invasive confirmatory biopsies. Here, we propose a less invasive method using an electrochemical DNA (E-DNA) biosensor for CD-specific autoantibodies (AABs) circulating in blood. In our approach, CD-specific AABs bind a synthetic neoepitope, causing a conformational change in the biosensor, as well as a change in the environment of an attached redox reporter, producing a measurable current reduction. We assessed the bi

SUBMITTER: Nguyen ABN 

PROVIDER: S-EPMC8070315 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature