Effect of Obesity on the Expression of Nutrient Receptors and Satiety Hormones in the Human Colon.

ABSTRACT:

Background

Receptors located on enteroendocrine cells (EECs) of the colon can detect nutrients in the lumen. These receptors regulate appetite through a variety of mechanisms, including hormonal and neuronal signals. We assessed the effect of obesity on the expression of these G-protein coupled receptors (GPCRs) and hormones at both mRNA and protein level.

Methods

qPCR and immunohistochemistry were used to examine colonic tissue from cohorts of patients from the Netherlands (proximal and sigmoid tissue) and the United Kingdom (tissue from across the colon) and patients were grouped by body mass index (BMI) value (BMI < 25 and BMI ≥ 25).

Results

The mRNA expression of the hormones/signaling molecules serotonin, glucagon, peptide YY (PYY), CCK and somatostatin were not

SUBMITTER: Baumard L 

PROVIDER: S-EPMC8070384 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature