Structural Biology for the Molecular Insight between Aptamers and Target Proteins.

ABSTRACT: Aptamers are promising therapeutic and diagnostic agents for various diseases due to their high affinity and specificity against target proteins. Structural determination in combination with multiple biochemical and biophysical methods could help to explore the interacting mechanism between aptamers and their targets. Regrettably, structural studies for aptamer-target interactions are still the bottleneck in this field, which are facing various difficulties. In this review, we first reviewed the methods for resolving structures of aptamer-protein complexes and for analyzing the interactions between aptamers and target proteins. We summarized the general features of the interacting nucleotides and residues involved in the interactions between aptamers and proteins. Challenges and perspectiv

SUBMITTER: Zhang N 

PROVIDER: S-EPMC8071422 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature