Structural and mechanical characterization of biofilm-associated bacterial polymer in the emulsification of petroleum hydrocarbon.

ABSTRACT: The marine bacterium Pseudomonas furukawaii PPS-19 isolated from the oil-polluted site of Paradip port, Odisha, India, was found to form a strong biofilm in 2% (v/v) crude oil. Confocal Laser Scanning Microscopy (CLSM) revealed biofilm components along with multi-layered dense biofilm of rod-shaped cells with 64.7 µm thickness. Scanning electron micrographs showed similar biofilm architecture covered with a gluey matrix of extracellular polymeric substances (EPS) in the presence of 2% (v/v) crude oil. The architecture of purified EPS was also studied through FESEM that exposed its porous and three-dimensional flakes-like structure. The structural characterization by FTIR revealed that EPS was composed of primary alkane, amines, halide, hydroxyl groups, uronic acid, and saccharides.

SUBMITTER: Vandana 

PROVIDER: S-EPMC8071786 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature