Single-cell visualization and quantification of trace metals in Chlamydomonas lysosome-related organelles.

ABSTRACT: The acidocalcisome is an acidic organelle in the cytosol of eukaryotes, defined by its low pH and high calcium and polyphosphate content. It is visualized as an electron-dense object by transmission electron microscopy (TEM) or described with mass spectrometry (MS)-based imaging techniques or multimodal X-ray fluorescence microscopy (XFM) based on its unique elemental composition. Compared with MS-based imaging techniques, XFM offers the additional advantage of absolute quantification of trace metal content, since sectioning of the cell is not required and metabolic states can be preserved rapidly by either vitrification or chemical fixation. We employed XFM in Chlamydomonas reinhardtii to determine single-cell and organelle trace metal quotas within algal cells in situations of trace meta

SUBMITTER: Schmollinger S 

PROVIDER: S-EPMC8072209 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature