Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers.

ABSTRACT: The gut epithelium serves to maximize the surface for nutrient and fluid uptake, but at the same time must provide a tight barrier to pathogens and remove damaged intestinal epithelial cells (IECs) without jeopardizing barrier integrity. How the epithelium coordinates these tasks remains a question of significant interest. We used imaging and an optical flow analysis pipeline to study the dynamicity of untransformed murine and human intestinal epithelia, cultured atop flexible hydrogel supports. Infection with the pathogen Salmonella Typhimurium (STm) within minutes elicited focal contractions with inward movements of up to ∼1,000 IECs. Genetics approaches and chimeric epithelial monolayers revealed contractions to be triggered by the NAIP/NLRC4 inflammasome, which sensed type-III secretio

SUBMITTER: Samperio Ventayol P 

PROVIDER: S-EPMC8072224 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature