Ontology highlight
ABSTRACT:
SUBMITTER: Chatterjee S
PROVIDER: S-EPMC8072259 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20210401 16
Bacterial messenger RNA (mRNA) synthesis by RNA polymerase (RNAP) and first-round translation by the ribosome are often coupled to regulate gene expression, yet how coupling is established and maintained is ill understood. Here, we develop biochemical and single-molecule fluorescence approaches to probe the dynamics of RNAP-ribosome interactions on an mRNA with a translational preQ<sub>1</sub>-sensing riboswitch in its 5' untranslated region. Binding of preQ<sub>1</sub> leads to the occlusion of ...[more]