Current Methods of Post-Translational Modification Analysis and Their Applications in Blood Cancers.

ABSTRACT: Post-translational modifications (PTMs) add a layer of complexity to the proteome through the addition of biochemical moieties to specific residues of proteins, altering their structure, function and/or localization. Mass spectrometry (MS)-based techniques are at the forefront of PTM analysis due to their ability to detect large numbers of modified proteins with a high level of sensitivity and specificity. The low stoichiometry of modified peptides means fractionation and enrichment techniques are often performed prior to MS to improve detection yields. Immuno-based techniques remain popular, with improvements in the quality of commercially available modification-specific antibodies facilitating the detection of modified proteins with high affinity. PTM-focused studies on blood cancers hav

SUBMITTER: Dunphy K 

PROVIDER: S-EPMC8072572 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature