Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells.

ABSTRACT: Owing to viral evolution and recombination, emerging pseudorabies virus (PRV) strains have caused unprecedented outbreaks in swine farms even when the pigs were previously vaccinated, which might indicate that traditional vaccines were unable to provide effective protection. The development of safe and efficacious vaccines presents prospects to minimize the clinical signs and eventually eradicate the infection. In this study, we used an emerging PRV strain, HNX, as the parental strain to construct a recombinant PRV with TK/gE gene deletion and Fms-related tyrosine kinase 3 ligand (Flt3L) expression, named HNX-TK^-/gE^--Flt3L. HNX-TK^-/gE^--Flt3L enhanced the maturation of bone marrow derived dendritic cells (DCs) in vitro. Significantly more activate

SUBMITTER: Yao L 

PROVIDER: S-EPMC8072707 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature