Genome-Wide Identification and Expression Analysis of SOS Response Genes in Salmonella enterica Serovar Typhimurium.

ABSTRACT: A bioinformatic search for LexA boxes, combined with transcriptomic detection of loci responsive to DNA damage, identified 48 members of the SOS regulon in the genome of Salmonella enterica serovar Typhimurium. Single cell analysis using fluorescent fusions revealed that heterogeneous expression is a common trait of SOS response genes, with formation of SOS^OFF and SOS^ON subpopulations. Phenotypic cell variants formed in the absence of external DNA damage show gene expression patterns that are mainly determined by the position and the heterology index of the LexA box. SOS induction upon DNA damage produces SOS^OFF and SOS^ON subpopulations that contain live and dead cells. The nature and concentration of the DNA damaging agent and the time of

SUBMITTER: Merida-Floriano A 

PROVIDER: S-EPMC8072944 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature