Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning.

ABSTRACT:

Background

The G-protein-coupled receptor GPR55 has been implicated in multiple biological activities, which has fuelled interest in its functional targeting. Its controversial pharmacology and often species-dependent regulation have impacted upon the potential translation of preclinical data involving GPR55.

Results

With the aim to identify novel GPR55 regulators, we have investigated lysophosphatidylinositol (LPI)-induced GPR55-mediated signal transduction. The expression system for wild-type and mutated GPR55 was HeLa cells silenced for their endogenous receptor by stable expression of a short-hairpin RNA specific for GPR55 5'-UTR, which allowed definition of the requirement of GPR55 Lys⁸⁰ for LPI-induced MAPK activation and receptor internalisation. In RAW264.

SUBMITTER: Mosca MG 

PROVIDER: S-EPMC8073907 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature