Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes.

ABSTRACT: There has been a recent increase in the exploration of cold-active β-galactosidases, as it offers new alternatives for the dairy industry, mainly in response to the current needs of lactose-intolerant consumers. Since extremophilic microbial compounds might have unique physical and chemical properties, this research aimed to study the capacity of Antarctic bacterial strains to produce cold-active β-galactosidases. A screening revealed 81 out of 304 strains with β-galactosidase activity. The strain Se8.10.12 showed the highest enzymatic activity. Morphological, biochemical, and molecular characterization based on whole-genome sequencing confirmed it as the first Rahnella inusitata isolate from the Antarctic, which retained 41-62% of its β-galactosidase activity in the cold (4 °C-15 °

SUBMITTER: Nunez-Montero K 

PROVIDER: S-EPMC8074230 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature