Aneuploid abortion correlates positively with MAD1 overexpression and miR-125b down-regulation.

ABSTRACT:

Background

Aneuploidy is the most frequent cause of early-embryo abortion. Any defect in chromosome segregation would fail to satisfy the spindle assembly checkpoint (SAC) during mitosis, halting metaphase and causing aneuploidy. The mitotic checkpoint complex (MCC), comprising MAD1, MAD2, Cdc20, BUBR1 and BUB3, plays a vital role in SAC activation. Studies have confirmed that overexpression of MAD2 and BUBR1 can facilitate correct chromosome segregation and embryo stability. Research also proves that miR-125b negatively regulates MAD1 expression by binding to its 3'UTR. However, miR-125b, Mad1 and Bub3 gene expression in aneuploid embryos of spontaneous abortion has not been reported to date.

Methods

In this study, embryonic villi from miscarried pregnancies were collected

SUBMITTER: Zhao J 

PROVIDER: S-EPMC8074413 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature