Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9.

ABSTRACT:

Background

Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. Accelerating the drug development process by simple, quick and scalable access of human drug metabolites is key for efficient and targeted drug development in response to new and sometimes unexpected medical challenges and needs. However, due its biochemical complexity, scalable human CYP (hCYP) production and their application in preparative biotransformations was still in its infancy.

Results

A scalable bioprocess for fine-tuned co-expression of hCYP2C9 and its essential complementary human cytochrome P450 reductase (hCPR) in the yeast Pichia pastoris (Komagataella phaffii

SUBMITTER: Garrigos-Martinez J 

PROVIDER: S-EPMC8074423 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature