Size-controlled human adipose-derived stem cell spheroids hybridized with single-segmented nanofibers and their effect on viability and stem cell differentiation.

ABSTRACT:

Background

Fabrication of three-dimensional stem cell spheroids have been studied to improve stem cell function, but the hypoxic core and limited penetration of nutrients and signaling cues to the interior of the spheroid were challenges. The incorporation of polymers such as silica and gelatin in spheroids resulted in relatively relaxed assembly of composite spheroids, and enhancing transport of nutrient and biological gas. However, because of the low surface area between cells and since the polymers were heterogeneously distributed throughout the spheroid, these polymers cannot increase the cell to extracellular matrix interactions needed to support differentiation.

Methods

We developed the stem cell spheroids that incorporate poly(ι-lactic acid) single-segmented fibers sy

SUBMITTER: Lee J 

PROVIDER: S-EPMC8074457 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature