Dense time-course gene expression profiling of the Drosophila melanogaster innate immune response.

ABSTRACT:

Background

Immune responses need to be initiated rapidly, and maintained as needed, to prevent establishment and growth of infections. At the same time, resources need to be balanced with other physiological processes. On the level of transcription, studies have shown that this balancing act is reflected in tight control of the initiation kinetics and shutdown dynamics of specific immune genes.

Results

To investigate genome-wide expression dynamics and trade-offs after infection at a high temporal resolution, we performed an RNA-seq time course on D. melanogaster with 20 time points post Imd stimulation. A combination of methods, including spline fitting, cluster analysis, and Granger causality inference, allowed detailed dissection of expression profiles, lead-lag interacti

SUBMITTER: Schlamp F 

PROVIDER: S-EPMC8074482 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature