Gas-Phase Protonation Thermodynamics of Biological Lipids: Experiment, Theory, and Implications.

ABSTRACT: Phospholipids are important to cellular function and are a vital structural component of plasma and organelle membranes. These membranes isolate the cell from its environment, allow regulation of the internal concentrations of ions and small molecules, and host diverse types of membrane proteins. It remains extremely challenging to identify specific membrane protein-lipid interactions and their relative strengths. Native mass spectrometry, an intrinsically gas-phase method, has recently been demonstrated as a promising tool for identifying endogenous protein-lipid interactions. However, to what extent the identified interactions reflect solution- versus gas-phase binding strengths is not known. Here, the "Extended" Kinetic Method and ab initio computations at three different levels

SUBMITTER: Miller ZM 

PROVIDER: S-EPMC8074629 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature