Porphyromonas gingivalis lipopolysaccharide promotes T-hel per17 cell differentiation by upregulating Delta-like ligand 4 expression on CD14⁺ monocytes.

ABSTRACT:

Backgroud

To investigate the effect and mechanism of Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) on Th17 cell differentiation mediated by CD14⁺ monocytes.

Methods

P. gingivalis LPS-activated CD14⁺ monocytes were co-cultured with CD4⁺T cells in different cell ratios. An indirect co-culture system was also established using transwell chambers. Furthermore, anti- Delta-like ligand 4 (Dll-4) antibody was used to investigate the role of Dll-4 in Th17 cell response. The mRNA expression was analyzed using quantitative reverse transcription-polymerase chain reaction, and secreted cytokines in culture supernatant were detected using enzyme-linked immunosorbent assay. Flow cytometry was used to determin

SUBMITTER: Zhang C 

PROVIDER: S-EPMC8074840 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature