An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures.

ABSTRACT: Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.

SUBMITTER: Ding R 

PROVIDER: S-EPMC8074886 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature