Comprehensive Quantification of Carboxymethyllysine-Modified Peptides in Human Plasma.

ABSTRACT: A prolonged hyperglycemic condition in diabetes mellitus results in glycation of plasma proteins. N(ε)-Carboxymethyllysine (CML) is a well-known protein advanced glycation end product, and one of its mechanisms of formation is through further oxidation of Amadori compound modified lysine (AML). Unlike enrichment of AML peptides using boronate affinity, biochemical enrichment methods are scarce for comprehensive profiling of CML-modified peptides. To address this problem, we used AML peptide sequence and site of modification as template library to identify and quantify CML peptides. In this study, a parallel reaction monitoring workflow was developed to comprehensively quantify CML modified peptides in Type 1 diabetic subjects' plasma with good and poor glycemic control (n = 2

SUBMITTER: Korwar AM 

PROVIDER: S-EPMC8075102 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature