Dataset Information

VolPy: Automated and scalable analysis pipelines for voltage imaging datasets.

ABSTRACT: Voltage imaging enables monitoring neural activity at sub-millisecond and sub-cellular scale, unlocking the study of subthreshold activity, synchrony, and network dynamics with unprecedented spatio-temporal resolution. However, high data rates (>800MB/s) and low signal-to-noise ratios create bottlenecks for analyzing such datasets. Here we present VolPy, an automated and scalable pipeline to pre-process voltage imaging datasets. VolPy features motion correction, memory mapping, automated segmentation, denoising and spike extraction, all built on a highly parallelizable, modular, and extensible framework optimized for memory and speed. To aid automated segmentation, we introduce a corpus of 24 manually annotated datasets from different preparations, brain areas and voltage indicators. We benchmark VolPy against ground truth segmentation, simulations and electrophysiology recordings, and we compare its performance with existing algorithms in detecting spikes. Our results indicate that VolPy's performance in spike extraction and scalability are state-of-the-art.

SUBMITTER: Cai C

PROVIDER: S-EPMC8075204 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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VolPy: Automated and scalable analysis pipelines for voltage imaging datasets.

Cai Changjia C Friedrich Johannes J Singh Amrita A Eybposh M Hossein MH Pnevmatikakis Eftychios A EA Podgorski Kaspar K Giovannucci Andrea A

PLoS computational biology 20210414 4

Voltage imaging enables monitoring neural activity at sub-millisecond and sub-cellular scale, unlocking the study of subthreshold activity, synchrony, and network dynamics with unprecedented spatio-temporal resolution. However, high data rates (>800MB/s) and low signal-to-noise ratios create bottlenecks for analyzing such datasets. Here we present VolPy, an automated and scalable pipeline to pre-process voltage imaging datasets. VolPy features motion correction, memory mapping, automated segment ...[more]

PMID: 33852574

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Dataset Information

VolPy: Automated and scalable analysis pipelines for voltage imaging datasets.

Publications

VolPy: Automated and scalable analysis pipelines for voltage imaging datasets.

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