Synchrotron X-ray micro-CT as a validation dataset for diffusion MRI in whole mouse brain.

ABSTRACT:

Purpose

To introduce synchrotron X-ray microcomputed tomography (microCT) and demonstrate its use as a natively isotropic, nondestructive, 3D validation modality for diffusion MRI in whole, fixed mouse brain.

Methods

Postmortem diffusion MRI and microCT data were acquired of the same whole mouse brain. Diffusion data were processed using constrained spherical deconvolution. Synchrotron data were acquired at an isotropic voxel size of 1.17 μm. Structure tensor analysis was used to calculate fiber orientation distribution functions from the microCT data. A pipeline was developed to spatially register the 2 datasets in order to perform qualitative comparisons of fiber geometries represented by fiber orientation distribution functions. Fiber orientations from both modalities wer

SUBMITTER: Trinkle S 

PROVIDER: S-EPMC8076078 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature