Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis.

ABSTRACT:

Background and aim

The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the Brucella organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against Yersinia enterocolitica O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of Brucella abortus for their diagnostic potential in cattle brucellosis.

Materials and methods

Protein antigens of B. abortus (n=10) non-reactive to antibodies against Y. enterocolitica O:9 were selected, expressed in Escherichia coli, assessed the reactivity of expressed recombinant proteins by Western blo

SUBMITTER: Nagalingam M 

PROVIDER: S-EPMC8076471 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature