Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris.

ABSTRACT:

Background

Protein synthesis is one of the extremely important anabolic pathways in the yeast expression system Pichia pastoris. Codon optimization is a commonly adopted strategy for improved protein expression, although unexpected failures did appear sometimes waiting for further exploration. Recently codon bias has been studied to regulate protein folding and activity in many other organisms.

Results

Here the codon bias profile of P. pastoris genome was examined first and a direct correlation between codon translation efficiency and usage frequency was identified. By manipulating the codon choices of both endogenous and heterologous signal peptides, secretion abilities of N-terminal signal peptides were shown to be tolerant towards codon changes. Then two gene candidates w

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8077831 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature