Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.

ABSTRACT: As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high. The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features. Therefore, well-characterized recombinant RBDs are essential. We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells. To structurally characterize the native RBDs (comprising N- and O-glycans and additional post tran

SUBMITTER: Gstottner C 

PROVIDER: S-EPMC8078197 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature