Determination of the vRNA and cRNA promoter activity by M segment-specific non-coding nucleotides of influenza A virus.

ABSTRACT: Eight-segmented, negative-sense, single-stranded genomic RNAs of influenza A virus are terminated with 5' and 3' untranslated regions (UTRs). All segments have highly conserved extremities of 13 and 12 nucleotides at the 5' and 3' UTRs, respectively, constructing the viral RNA (vRNA) promoter. Adjacent to the duplex stem of 3 base pairs (bps) between the two conserved strands, additional 1-4 bps are existing in a segment-specific manner. We investigated the roles of the matrix (M) segment-specific base pair between the 14^th nucleotide uridine (U14') of the 5' UTR and the 13^th nucleotide adenosine (A13) of the 3' UTR by preparing possible vRNA promoters, named vXY, as well as cRNA promoters, named cYX. We analysed their RNA-dependent RNA replication efficiency using th

SUBMITTER: Shin H 

PROVIDER: S-EPMC8078515 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature