Molecular determinants and heterogeneity underlying host response to EV-A71 infection at single-cell resolution.

ABSTRACT: The pathogenic human enterovirus EV-A71 has raised serious public health concerns. A hallmark of EV-A71 infection is the distortion of host transcriptomes in favour of viral replication. While high-throughput approaches have been exploited to dissect these gene dysregulations, they do not fully capture molecular perturbations at the single-cell level and in a physiologically relevant context. In this study, we applied a single-cell RNA sequencing approach on infected differentiated enterocyte cells (C2BBe1), which model the gastrointestinal epithelium targeted initially by EV-A71. Our single-cell analysis of EV-A71-infected culture provided several lines of illuminating observations: 1) This systems approach demonstrated extensive cell-to-cell variation in a single culture upon viral infec

SUBMITTER: Kuo RL 

PROVIDER: S-EPMC8078531 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature