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Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences.


ABSTRACT: Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three Rubus accessions (two Rubus longisepalus and one R. hirsutus) were newly assembled using Illumina whole-genome sequences. Rubus longisepalus Nakai and R. longisepalus var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in R. longisepalus and R. hirsutus, respectively. The 5S rDNA transcription unit is an identical 121 bp in three Rubus accessions. We developed three DNA markers to authenticate R. longisepalus and R. hirsutus based on plastome diversity. Phylogenomic analysis revealed that the Rubus species classified as two clades and R. longisepalus, R. hirsutus, and R. chingii are the most closely related species in clade 1.

SUBMITTER: Park YS 

PROVIDER: S-EPMC8079122 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Diversity and authentication of <i>Rubus</i> accessions revealed by complete plastid genome and rDNA sequences.

Park Young Sang YS   Park Jee Young JY   Kang Jung Hwa JH   Lee Wan Hee WH   Yang Tae-Jin TJ  

Mitochondrial DNA. Part B, Resources 20210420 4


Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three <i>Rubus</i> accessions (two <i>Rubus longisepalus</i> and one <i>R. hirsutus</i>) were newly assembled using Illumina whole-genome sequences. <i>Rubus longisepalus</i> Nakai and <i>R. longisepalus</i> var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in <i>R. longise  ...[more]

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