A Virulent PEDV Strain FJzz1 with Genomic Mutations and Deletions at the High Passage Level Was Attenuated in Piglets via Serial Passage In Vitro.

ABSTRACT: Highly virulent porcine epidemic diarrhea virus (PEDV) strains re-emerged and circulated in China at the end of 2010, causing significant economic losses in the pork industry worldwide. To understand the genetic dynamics of PEDV during its passage in vitro, the PEDV G2 strain FJzz1 was serially propagated in Vero cells for up to 200 passages. The susceptibility and adaptability of the FJzz1 strain increased gradually as it was serially passaged in vitro. Sequence analysis revealed that amino acid (aa) changes were mainly concentrated in the S glycoprotein, which accounted for 72.22%-85.71% of all aa changes. A continuous aa deletion (⁵⁵I⁵⁶G⁵⁷E → ⁵⁵K⁵⁶Δ⁵⁷Δ) occurred in the N-terminal domain of S1 (S1-NTD). To examine how the

SUBMITTER: Chen P 

PROVIDER: S-EPMC8080196 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature