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Early satellite cell communication creates a permissive environment for long-term muscle growth.

ABSTRACT: Using in vivo muscle stem cell (satellite cell)-specific extracellular vesicle (EV) tracking, satellite cell depletion, in vitro cell culture, and single-cell RNA sequencing, we show satellite cells communicate with other cells in skeletal muscle during mechanical overload. Early satellite cell EV communication primes the muscle milieu for proper long-term extracellular matrix (ECM) deposition and is sufficient to support sustained hypertrophy in adult mice, even in the absence of fusion to muscle fibers. Satellite cells modulate chemokine gene expression across cell types within the first few days of loading, and EV delivery of miR-206 to fibrogenic cells represses Wisp1 expression required for appropriate ECM remodeling. Late-stage communication from myogenic cells during loading is widespread but may be targeted toward endothelial cells. Satellite cells coordinate adaptation by influencing the phenotype of recipient cells, which extends our understanding of their role in muscle adaptation beyond regeneration and myonuclear donation.

SUBMITTER: Murach KA

PROVIDER: S-EPMC8080523 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Early satellite cell communication creates a permissive environment for long-term muscle growth.

Murach Kevin A KA Peck Bailey D BD Policastro Robert A RA Vechetti Ivan J IJ Van Pelt Douglas W DW Dungan Cory M CM Denes Lance T LT Fu Xu X Brightwell Camille R CR Zentner Gabriel E GE Dupont-Versteegden Esther E EE Richards Christopher I CI Smith Jeramiah J JJ Fry Christopher S CS McCarthy John J JJ Peterson Charlotte A CA

iScience 20210329 4

Using <i>in vivo</i> muscle stem cell (satellite cell)-specific extracellular vesicle (EV) tracking, satellite cell depletion, <i>in vitro</i> cell culture, and single-cell RNA sequencing, we show satellite cells communicate with other cells in skeletal muscle during mechanical overload. Early satellite cell EV communication primes the muscle milieu for proper long-term extracellular matrix (ECM) deposition and is sufficient to support sustained hypertrophy in adult mice, even in the absence of ...[more]

PMID: 33948557

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Project description:This study investigated the effects of long-term serum starvation on autophagy, metabolism, and differentiation of porcine skeletal muscle satellite cells (SMSCs) and elucidated the role of autophagy in skeletal muscle development. Our findings provide a theoretical basis for improving meat production in domestic pigs. The SMSCs isolated and preserved in our laboratory were revived and divided into six groups based on the culture medium serum concentration to simulate varying levels of serum starvation: 20% serum (control group), 15% serum (mild serum starvation group), 5% serum (severe serum starvation group), and their autophagy inhibition groups supplemented with 3-methyladenine. After 96 h of culture, the apoptosis rate, mitochondrial membrane potential, reactive oxygen species, and ATP were measured to evaluate the effects of serum starvation on the SMSCs' metabolism. Additionally, the levels of autophagy-related proteins, autophagosomes, and autolysosomes were measured to investigate the impact of long-term serum starvation on autophagy. The expression of proteins associated with myogenic and adipogenic differentiation (MHC, MyoD1, peroxisome proliferator-activated receptor γ, and lipoprotein lipase) as well as lipid content were also determined to investigate the effects of long-term serum starvation on SMSC differentiation. The results showed that long-term serum starvation induced autophagy through the AMPK/mTOR signaling pathway, accelerated cell metabolism and apoptosis, exacerbated reactive oxygen species accumulation, and inhibited myogenic and adipogenic differentiation of SMSCs. Moreover, these effects were positively correlated with the level of serum starvation. In addition, serum starvation-induced autophagy moderately promoted the myogenic and adipogenic differentiation of SMSCs; however, these effects were insufficient to counteract the inhibition of cell differentiation by long-term serum starvation. This study provides insight into leveraging serum starvation as a stressor to regulate muscle growth and metabolism in domestic pigs.

Dataset Information

Early satellite cell communication creates a permissive environment for long-term muscle growth.

Publications

Early satellite cell communication creates a permissive environment for long-term muscle growth.

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