The mRNA derived MalH sRNA contributes to alternative carbon source utilization by tuning maltoporin expression in E. coli.

ABSTRACT: Previous high-throughput studies in Gram-negative bacteria identified a large number of 3'UTR fragments that potentially function as sRNAs. Here we extensively characterize the MalH sRNA. We show that MalH is a stable degradation intermediate derived from the 3' end of malG, which is part of the maltose uptake operon transcript malEFG. Unlike the majority of bacterial sRNAs, MalH is transiently expressed during the transition from the exponential to the stationary growth phase, suggesting that it contributes to adaptation to changes in nutrient availability. Over-expression of MalH reduces expression of general outer membrane porins and MicA, a repressor of the high-affinity maltose/maltodextrin transporter LamB. Disrupting MalH production and function significantly reduces <

SUBMITTER: Iosub IA 

PROVIDER: S-EPMC8081044 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature