Acetate Induces Growth Arrest in Colon Cancer Cells Through Modulation of Mitochondrial Function.

ABSTRACT: Acetate is one of the main short chain fatty acids produced in the colon when fermentable carbohydrates are digested. It has been shown to affect normal metabolism, modulating mitochondrial function, and fatty acid oxidation. Currently, there is no clear consensus regarding the effects of acetate on tumorigenesis and cancer metabolism. Here, we investigate the metabolic effects of acetate on colon cancer. HT29 and HCT116 colon cancer cell lines were treated with acetate and its effect on mitochondrial proliferation, reactive oxygen species, density, permeability transition pore, cellular bioenergetics, gene expression of acetyl-CoA synthetase 1 (ACSS1) and 2 (ACSS2), and lipid levels were investigated. Acetate was found to reduce proliferation of both cell lines under normoxi

SUBMITTER: Sahuri-Arisoylu M 

PROVIDER: S-EPMC8081909 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature