Rational affinity maturation of anti-amyloid antibodies with high conformational and sequence specificity.

ABSTRACT: The aggregation of amyloidogenic polypeptides is strongly linked to several neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. Conformational antibodies that selectively recognize protein aggregates are leading therapeutic agents for selectively neutralizing toxic aggregates, diagnostic and imaging agents for detecting disease, and biomedical reagents for elucidating disease mechanisms. Despite their importance, it is challenging to generate high-quality conformational antibodies in a systematic and site-specific manner due to the properties of protein aggregates (hydrophobic, multivalent, and heterogeneous) and limitations of immunization (uncontrolled antigen presentation and immunodominant epitopes). Toward addressing these challenges, we have developed a syste

SUBMITTER: Desai AA 

PROVIDER: S-EPMC8081927 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature