Myosin V Regulates Spatial Localization of Different Forms of Neurotransmitter Release in Central Synapses.

ABSTRACT: Synaptic active zone (AZ) contains multiple specialized release sites for vesicle fusion. The utilization of release sites is regulated to determine spatiotemporal organization of the two main forms of synchronous release, uni-vesicular (UVR) and multi-vesicular (MVR). We previously found that the vesicle-associated molecular motor myosin V regulates temporal utilization of release sites by controlling vesicle anchoring at release sites in an activity-dependent manner. Here we show that acute inhibition of myosin V shifts preferential location of vesicle docking away from AZ center toward periphery, and results in a corresponding spatial shift in utilization of release sites during UVR. Similarly, inhibition of myosin V also reduces preferential utilization of central release sites during

SUBMITTER: Maschi D 

PROVIDER: S-EPMC8081987 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature