Three-in-One Simultaneous Extraction of Proteins, Metabolites and Lipids for Multi-Omics.

ABSTRACT: Elucidation of complex molecular networks requires integrative analysis of molecular features and changes at different levels of information flow and regulation. Accordingly, high throughput functional genomics tools such as transcriptomics, proteomics, metabolomics, and lipidomics have emerged to provide system-wide investigations. Unfortunately, analysis of different types of biomolecules requires specific sample extraction procedures in combination with specific analytical instrumentation. The most efficient extraction protocols often only cover a restricted type of biomolecules due to their different physicochemical properties. Therefore, several sets/aliquots of samples are needed for extracting different molecules. Here we adapted a biphasic fractionation method to extract proteins,

SUBMITTER: Kang J 

PROVIDER: S-EPMC8082496 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature