AQP8 is a crucial H₂O₂ transporter in insulin-producing RINm5F cells.

ABSTRACT: Peroxiporins are distinct aquaporins (AQP) which, beside water, also facilitate the bidirectional transport of hydrogen peroxide (H₂O₂) across cellular membranes. H₂O₂ serves as the major reactive oxygen species that mediates essential cell signaling events. In pancreatic β-cells, H₂O₂ has been associated with the regulation of cell growth but in excess it leads to failure of insulin secretion, making it important for diabetes mellitus (DM) pathogenesis. In the present study, the role of aquaporin-8 (AQP8) as a peroxiporin was investigated in RINm5F cells. The role of AQP8 was studied in an insulin-producing cell model, on the basis of stable AQP8 overexpression (AQP8↑) and CRISPR/Cas9-mediated AQP8 knockdown (KD). A complet

SUBMITTER: Kruger C 

PROVIDER: S-EPMC8082690 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature