Impact of immediate cryopreservation on the establishment of patient derived xenografts from head and neck cancer patients.

ABSTRACT:

Background

Patient-derived xenografts established from human cancers are important tools for investigating novel anti-cancer therapies. Establishing PDXs requires a significant investment and many PDXs may be used infrequently due to their similarity to existing models, their growth rate, or the lack of relevant mutations. We performed this study to determine whether we could efficiently establish PDXs after cryopreservation to allow molecular profiling to be completed prior to implanting the human cancer.

Methods

Fresh tumor was split with half used to establish a PDX immediately and half cryopreserved for later implantation. Resulting tumors were assessed histologically and tumors established from fresh or cryopreserved tissues compared as to the growth rate, extent of tum

SUBMITTER: Abel L 

PROVIDER: S-EPMC8082827 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature