N-terminal heterogeneity of parenchymal and vascular amyloid-β deposits in Alzheimer's disease.

ABSTRACT:

Aims

The deposition of amyloid-β (Aβ) peptides in the form of extracellular plaques in the brain represents one of the classical hallmarks of Alzheimer's disease (AD). In addition to 'full-length' Aβ starting with aspartic acid (Asp-1), considerable amounts of various shorter, N-terminally truncated Aβ peptides have been identified by mass spectrometry in autopsy samples from individuals with AD.

Methods

Selectivity of several antibodies detecting full-length, total or N-terminally truncated Aβ species has been characterized with capillary isoelectric focusing assays using a set of synthetic Aβ peptides comprising different N-termini. We further assessed the N-terminal heterogeneity of extracellular and vascular Aβ peptide deposits in the human brain by performing immunohist

SUBMITTER: Zampar S 

PROVIDER: S-EPMC8082844 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature