Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility.

ABSTRACT:

Background

Vertebrate meiotic recombination events are concentrated in regions (hotspots) that display open chromatin marks, such as trimethylation of lysines 4 and 36 of histone 3 (H3K4me3 and H3K36me3). Mouse and human PRDM9 proteins catalyze H3K4me3 and H3K36me3 and determine hotspot positions, whereas other vertebrates lacking PRDM9 recombine in regions with chromatin already opened for another function, such as gene promoters. While these other vertebrate species lacking PRDM9 remain fertile, inactivation of the mouse Prdm9 gene, which shifts the hotspots to the functional regions (including promoters), typically causes gross fertility reduction; and the reasons for these species differences are not clear.

Results

We introduced Prdm9 deletions into the Rattus norvegicus

SUBMITTER: Mihola O 

PROVIDER: S-EPMC8082845 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature