Pro-inflammatory effects of human apatite crystals extracted from patients suffering from calcific tendinopathy.

ABSTRACT:

Background

Calcific tendonitis of the rotator cuff is due to carbonated apatite deposits in the shoulder tendons. During the evolution of the disease, an acute inflammatory episode may occur leading to the disappearance of the calcification. Although hydroxyapatite crystal-induced inflammation has been previously studied with synthetic crystals, no data are available with calcifications extracted from patients suffering from calcific tendinopathy. The objective of the study was to explore the inflammatory properties of human calcifications and the pathways involved.

Methods

Human calcifications and synthetic hydroxyapatite were used in vitro to stimulate human monocytes and macrophages, the human myeloid cell line THP-1, and human tenocytes. The release of IL-1β, IL-6, and I

SUBMITTER: Herman J 

PROVIDER: S-EPMC8082912 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature