Intravital Imaging of Circulating Red Blood Cells in the Retinal Vasculature of Growing Mice.

ABSTRACT:

Purpose

To establish a custom-built, high-speed 90 frame-per-second laser-scanning confocal microscope for real-time in vivo retinal imaging of individual flowing red blood cells (RBCs) in retinal vasculature of live mouse model.

Methods

Fluorescently labeled RBCs were injected into mice of different ages (3 to 62 weeks old). Anti-CD31 antibody conjugated with Alexa Fluor 647 was injected to visualize retinal endothelial cells (ECs). Longitudinal and cross-sectional intravital retinal imaging of flowing RBCs and ECs was performed in two strains (C57BL/6 and Balb/c) by using the custom-built confocal microscope.

Results

Simultaneous tracking of the routes of many fluorescently labeled individual RBCs flowing from a large artery and vein to a single capillary in the ret

SUBMITTER: Jeon J 

PROVIDER: S-EPMC8083064 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature