Dataset Information

¹H, ¹³C, ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

ABSTRACT: The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5'-untranslated region (5'-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive ¹H, ¹³C, ¹⁵N and ³¹P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.

SUBMITTER: Vogele J

PROVIDER: S-EPMC8083917 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

Vögele Jennifer J Ferner Jan-Peter JP Altincekic Nadide N Bains Jasleen Kaur JK Ceylan Betül B Fürtig Boris B Grün J Tassilo JT Hengesbach Martin M Hohmann Katharina F KF Hymon Daniel D Knezic Bozana B Löhr Frank F Peter Stephen A SA Pyper Dennis D Qureshi Nusrat S NS Richter Christian C Schlundt Andreas A Schwalbe Harald H Stirnal Elke E Sudakov Alexey A Wacker Anna A Weigand Julia E JE Wirmer-Bartoschek Julia J Wöhnert Jens J Duchardt-Ferner Elke E

Biomolecular NMR assignments 20210429 2

The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5'- and 3'-g ...[more]

PMID: 33928512

Dataset Information

¹H, ¹³C, ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

Publications

<sup>1</sup>H, <sup>13</sup>C, <sup>15</sup>N and <sup>31</sup>P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

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