Residual immune response towards decellularized homografts may be highly individual.

ABSTRACT:

Objectives

Decellularized homograft valves (DHVs) have shown promising clinical results, particularly in the treatment of congenital heart disease. However, DHV appears to elicit an immune response in a subset of young patients, indicated by early valve degeneration. As the decellularization process is quality controlled for each DHV, we hypothesized that there may be residual immunogenicity within the extracellular matrix of DHV.

Methods

A semi-quantitative dot blot analysis was established to screen for preformed recipient antibodies using secondary anti-human antibodies. Fifteen DHV samples (7 aortic, 8 pulmonary) were solubilized and exposed to serum from 20 healthy controls.

Results

The sera from young controls (n = 10, 18-25 years) showed significantly stronger

SUBMITTER: Ebken J 

PROVIDER: S-EPMC8083949 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature