Comparison of Bone Turnover Biomarkers in Serum and Urine Measured on an Automated Analytical Platform.

ABSTRACT:

Background

Matched serum and urine samples from patients who had total hip replacement were used to assess serum-validated immunoassay reagents for use in urine.

Methods

Samples were evaluated by an automated electrochemiluminescent immunoassay (cobas e411; Roche Diagnostics) for C-terminal telopeptide of type I collagen isoform β (β-Crosslaps), osteocalcin N-terminal midfragment (N-MID OC), N-terminal propeptide of type I collagen (PINP), and interleukin 6 (IL-6). Spike and recovery experiments were utilized to assess urinary matrix effects. Correlations between serum and both raw and creatinine-corrected urinary measures were assessed. Short-term precision was assessed.

Results

Spike and recovery experiments indicated minimal matrix effects of urine for the β-Crossl

SUBMITTER: Wilson BM 

PROVIDER: S-EPMC8085578 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature