Unraveling the Extracellular Metabolism of Immortalized Hippocampal Neurons Under Normal Growth Conditions.

ABSTRACT: Metabolomic profiling of cell lines has shown many potential applications and advantages compared to animal models and human subjects, and an accurate cellular metabolite analysis is critical to understanding both the intracellular and extracellular environments in cell culture. This study provides a fast protocol to investigate in vitro metabolites of immortalized hippocampal neurons HN9.10e with minimal perturbation of the cell system using a targeted approach. HN9.10e neurons represent a reliable model of one of the most vulnerable regions of the central nervous system. Here, the assessment of their extracellular metabolic profile was performed by studying the cell culture medium before and after cell growth under standard conditions. The targeted analysis was performed by a dire

SUBMITTER: Campanella B 

PROVIDER: S-EPMC8085660 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature